تفاصيل الوثيقة
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نوع الوثيقة |
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مقال في مجلة دورية |
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عنوان الوثيقة |
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Enhancement of pathologist's routine practice: reuse of DNA extracted from immunostained formalin-fixed paraffin-embedded (FFPE) slides in downstream molecular analysis of cancer Enhancement of pathologist's routine practice: reuse of DNA extracted from immunostained formalin-fixed paraffin-embedded (FFPE) slides in downstream molecular analysis of cancer |
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لغة الوثيقة |
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الانجليزية |
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المستخلص |
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Background/Aim: To date, the conventional
formalin-fixed, paraffin-embedded (FFPE) technique is the
gold-standard for preserving histomorphology. Once FFPE
tissues are stained, slides are routinely archived along with
their blocks at biobanks/hospitals. However, the reuse of
fixed and stained biospecimens as DNA source is not a
common routine practice worldwide and, thus, indicates the
need of studies to investigate the feasibility of extracting
DNA from already immunohistochemistry (IHC) FFPEstained
slides and its possible reuse in subsequent
downstream molecular analyses. Materials and Methods:
FFPE IHC slides from colorectal cancer (CRC) patients
were prepared and stored in the CEGMR Biobank. The
workflow consists of digitalization of IHC stained slide’s
image, removing the slide cover-slip, crude dissection and
DNA extraction. Following DNA quality assessment,
mutation analysis of CTNNB1 and methylation profile of
CDH1 were performed. Results: High-quality DNA was
obtained allowing 60% concordance between CDH1
methylation and membranous E-cadherin expression pattern.
Clean CTNNB1 DNA chromatograms with evenly-spaced
peaks were observed. Conclusion: This study is a proof of
concept to recycle and reuse DNA from IHC stained slides
with suitable concentration and integrity for further
downstream molecular applications. These findings will
enhance the pathologists’ knowledge, attitudes and practices
(KAP) towards the use of these biospecimens and support the
implementation of this approach in clinical pathology
practice. Therefore, the scientific community will benefit
from the largest comprehensive database of human fully
annotated FFPE biospecimens already available at their
disposal in order to demystify the complexity and the
heterogeneity of many challenging diseases and foster the
transition towards precision medicine. |
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ردمد |
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1109-6535 |
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اسم الدورية |
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Cancer Genomics-Proteomics |
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المجلد |
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13 |
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العدد |
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5 |
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سنة النشر |
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1437 هـ
2016 م |
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نوع المقالة |
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مقالة علمية |
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تاريخ الاضافة على الموقع |
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Wednesday, July 12, 2017 |
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الباحثون
| ASMAA AL-ATTAS | AL-ATTAS, ASMAA | باحث | دكتوراه | |
| MOURAD ASSIDI | ASSIDI, MOURAD | باحث | دكتوراه | |
| JAUDAH AL-MAGHRABI | AL-MAGHRABI, JAUDAH | باحث | دكتوراه | |
| ASHRAF DALLOL | DALLOL, ASHRAF | باحث | دكتوراه | |
| ADEL ABUZENADAH | ABUZENADAH, ADEL | باحث | دكتوراه | |
| BRUCE BUDOWLE | BUDOWLE, BRUCE | باحث | دكتوراه | |
| HANS-JUERGEN SCHULTEN | SCHULTEN, HANS-JUERGEN | باحث | دكتوراه | |
| MUHAMMAD ABU-ELMAGD | ABU-ELMAGD, MUHAMMAD | باحث | دكتوراه | |
| ADEEL CHAUDHARY | CHAUDHARY, ADEEL | باحث | دكتوراه | |
| ABDELBASET BUHMEIDA | BUHMEIDA, ABDELBASET | باحث | دكتوراه | |
| MOHAMMED Al-Qahtani | AL-QAHTANI, MOHAMMED | باحث | دكتوراه | |
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الرجوع إلى صفحة الأبحاث
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